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micro fasttrack microfasttrack kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher micro fasttrack microfasttrack kit
    Micro Fasttrack Microfasttrack Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/micro+fasttrack+microfasttrack+kit/us07332298-171-26-35
    Average 86 stars, based on 1 article reviews
    micro fasttrack microfasttrack kit - by Bioz Stars, 2026-09
    86/100 stars

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    Isolation:

    Article Title: Nucleic acids encoding multimeric proteins of TNF superfamily ligands
    Article Snippet: .. Spleen cells from C3H/HeJ mice were stimulated with 5 μg/ml concanavalin A and 10 ng/ml IL-2 (Sigma) for 8 hours (31). mRNA was isolated using the Micro FastTrack MICROFASTTRACK kit (reagents for isolating messenger RNA, Invitrogen). cDNA was prepared using SUPERSCRIPT II reverse transcriptase (Life Technologies) and random hexamers as primers. .. PCR primer sequences were as follows (where the underlined bases indicate restriction endonuclease sites for cloning into the vector): 5mRANKL-ext: 5′-CATGTTCCTGGCCCTCCTC-3′ (SEQ ID NO:18) 3mRANKL-ext: 5′-GTACAGGCTCAAGAGAGAGGGC-3′ (SEQ ID NO:19) 5mRANKL-int: 5′-ATACTCGAGCGCAGATGGATCCTAAC-3′ (SEQ ID NO:20) 3mRANKL-int: 5′-GGGGTTTAGCGGCCGCTAATGTTCCACGAAATGAGTTC-3 (SEQ ID NO:21) The extracellular portion of RANKL/TRANCE was cloned by nested PCR.

    Reverse Transcription:

    Article Title: Nucleic acids encoding multimeric proteins of TNF superfamily ligands
    Article Snippet: .. Spleen cells from C3H/HeJ mice were stimulated with 5 μg/ml concanavalin A and 10 ng/ml IL-2 (Sigma) for 8 hours (31). mRNA was isolated using the Micro FastTrack MICROFASTTRACK kit (reagents for isolating messenger RNA, Invitrogen). cDNA was prepared using SUPERSCRIPT II reverse transcriptase (Life Technologies) and random hexamers as primers. .. PCR primer sequences were as follows (where the underlined bases indicate restriction endonuclease sites for cloning into the vector): 5mRANKL-ext: 5′-CATGTTCCTGGCCCTCCTC-3′ (SEQ ID NO:18) 3mRANKL-ext: 5′-GTACAGGCTCAAGAGAGAGGGC-3′ (SEQ ID NO:19) 5mRANKL-int: 5′-ATACTCGAGCGCAGATGGATCCTAAC-3′ (SEQ ID NO:20) 3mRANKL-int: 5′-GGGGTTTAGCGGCCGCTAATGTTCCACGAAATGAGTTC-3 (SEQ ID NO:21) The extracellular portion of RANKL/TRANCE was cloned by nested PCR.



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    micro fasttrack microfasttrack kit  (Thermo Fisher)
    86
    Thermo Fisher micro fasttrack microfasttrack kit
    Micro Fasttrack Microfasttrack Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/micro+fasttrack+microfasttrack+kit/us07332298-171-26-35
    Average 86 stars, based on 1 article reviews
    micro fasttrack microfasttrack kit - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

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